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KMID : 1005220090240020077
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Journal of Embryo Transfer
2009 Volume.24 No. 2 p.77 ~ p.87
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Comparative Analysis of Gene Expression in the Female Reproductive Organs
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Kim Min-Goo
Seo Hee-Won
Choi Yo-Han
Lee Chang-Kyu
Ka Hak-Hyun
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Abstract
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To understand molecular and cellular mechanisms of many gene products in the female reproductive organs including the ovary and uterine endometrium as well as during embryo development, researchers have developed and utilized many effective methodologies to analyze gene expression in cells, tissues and animals over the last several decades. For example, blotting techniques have helped to understand molecular functions at DNA, RNA and protein levels, and the reverse transcription-polymerase chain reaction (RT-PCR) method has been widely used in gene expression analysis. However, some conventional methods are not sufficient to understand regulation and function of genes expressed in very complex patterns in many organs. Thus, it is required to adopt more high-throughput and reliable techniques. Here, we describe several techniques used widely recently to analyze gene expression, including annealing control based-PCR, differential display-PCR, expressed sequence tag, suppression subtractive hybridization and microarray techniques. Use of these techniques will help to analyze expression pattern of many genes from small scale to large scale and to compare expression patterns of genes in one sample to another. In this review, we described principles of these methodologies and summarized examples of comparative analysis of gene expression in female reproductive organs with help of those methodologies.
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KEYWORD
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microarray, real-time RT-PCR, suppression subtractive hybridization, differential display PCR
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